英国论文：siRNA多种治疗方法的建立分析
多种治疗方法与各种机制相结合可以成为有效治疗具有联合或协同影响的癌症的可行选择。RNA的小干扰是一种强有力的技术，它是一种可行的治疗选择，可以沉默针对许多疾病的基因。基于siRNA的多种治疗方法已经建立起来，可以有效地治疗疾病。然而，由于涉及到负电荷，siRNA中细胞膜不易穿越，下游效应有延迟。这与传统的基于蛋白质或小分子的治疗方法相比较。
英国论文：siRNA多种治疗方法的建立分析
要成功地应用siRNA在临床上，就需要有效地传递载体。这对于克服细胞内的一些障碍是至关重要的。此外，与siRNA相比，小分子抗癌药物的效果更快。这在细胞内摄取的过程中是特定的。同时，纳米载体将瞄准药物，同时降低副作用。因此，一套基于siRNA的传统化疗程序的新疗法是具有优势的。
迄今为止，在硅、脂质体和基于聚合物的阳离子NPs的基础上建立了一些有前途的共交体系。与阳离子脂质相比，基于聚合物的非病毒载体具有较大的优势。这些优点具有生理稳定性好、生产规模大、方便安全等优点。对几种天然和合成阳离子聚合物作为siRNA或基因载体进行了研究。它们包括壳聚糖、多赖氨酸和聚乙烯亚胺。
英国论文：siRNA多种治疗方法的建立分析
The combination of several therapeutic approaches with various mechanisms can be feasible option for effectively treating cancers with combined or synergistic impacts. Small interference of RNA was a strong technique with a feasible therapeutic option to silence genes targeted in a number of diseases. A number of different therapeutics based on siRNA have established for treatment of diseases effectively. However, there cannot be easy crossing of cell membranes in siRNA due to the involvement of negative charge and there is a delay in downstream effects. This is in comparison with the conventional therapeutics based on protein or small molecule.
英国论文：siRNA多种治疗方法的建立分析
for being successful in clinically applying siRNA, there is a requirement to be effective in delivering vehicles. This is crucial for overcoming several barriers in the cell. In addition, anti-cancer drug effect of small molecule is fast in comparison with siRNA. This is specific in the process of intra-cellular uptake. Simultaneously, nanocarriers will be targeting the drugs while lowering the side effect. Therefore, the set of newly emerging therapy based on siRNA with traditional procedures of chemotherapy can be considered advantageous.
Until yet, a number of promising systems for the purposes of co-delivery are established on the basis of silica, liposomal and polymeric based cationic NPs. When compared, non-viral vectors based on polymer have major benefits in comparison with cationic lipids. These advantages are specific to physiological stability, large scale production, convenience and safety. There has been an investigation of several natural and synthetic cationic polymers as carriers of siRNA or gene. These include chitosan, polylysine and polyethyleneimine.
