代写论文：化学
在试验中，从6名患者中获得的分离物确定了新感染的隐球菌，这些患者接受了5 -氟- cytosin(5 – fc)治疗失败的治疗。研究发现，在治疗后，分离物表现出巨大且稳定的5 – fc阻力。提取的六种分离物中的五种表明对5 -氟尿嘧啶(5 -氟尿嘧啶)的巨大抗性。化合物是突变被认为是基于uridine-5′一磷酸焦磷酸化酶负责耐药性。对5 -氟胞嘧啶敏感的第6个分离物表明，复方CyH3或5 – fc1的摄取或合并没有减少。这种化合物的耐药机理还没有完全理解。然而，根据研究数据，存在几种可能性。这些类似于之前的研究报告中的upstype 2突变体。后者分离了5 – fc和5 – fu两种化合物的抗性。这些都说明了研究的原因。在三氯乙酸三氯乙酸中，它没有对4c标记的胞嘧啶的加入有任何损伤。在尿苷激酶内，强调了联合缺陷。这是一种酶催化尿苷的转化。
代写论文：化学
UMPase是在ups -type 2突变体中观察到的耐药性的模式。另一种解释是基于这个事实，5 – fc被转化为5 -氟- 2′ – deoxyuridine – 5′ -磷酸二磷酸(5f – dump)在52R。它们选择性地与胸腺肽酶(TS)结合。这与核苷酸连接中的RNA不相结合。5f – dump是一种非竞争性的抑制剂，它可以分解脱氧酸对胸腺酸的甲基化过程。这抑制了DNA的合成。以三氯乙酸为原料的5f – dump酶复合物被用于刺激t – novo合成，这使得DNA合成可以继续进行。这以较低的速率发生。这阻止了5 – fc的动作。在5 – fc的存在下，52R的增长率下降，这一假设得到了支持。第三种解释是考虑到5 – fc中52R的耐药性。从分析来看，它被发现被纳入RNA或52R化合物。这些并没有对细胞功能造成重大改变。这些情况都是可能的。5- fc或5 – fu复合物可通过52R进行除氟，可在三胆酸馏分中，对其耐药，并出现在放射性物质中。这些机制是遥远的，并没有微生物系统的先例。研究表明，基于病原体与抗真菌剂之间的相互作用，细胞会产生可能的影响。我们必须明白，在6个孤立的国家中，5个孤立的国家已经确定了具体的影响。第六分离也解释了这些关系的影响。在现实中，结构的形成是无法确定的。在研究的基础上可以开发出一个广阔的视角。
代写论文：化学
In the test, the isolates were obtained from six patients determined the Cryptococcus neoformans from patients who had an unsuccessful therapy with the treatment of 5-fluorocytosine (5-FC). It was found that after the therapy, the isolates were exhibiting massive and stable 5-FC resistance. The five of the six isolates that was extracted indicated that the massive resistance to the 5-fluorouracil (5-FU). It was deemed that the compound was the mutation was based on the uridine-5′-monophosphate pyrophosphorylase were responsible for the drug resistance. The 6th isolate that was susceptible to the 5-fluorocytosine indicated that there was no reduction in the uptake or incorporation of the compound CyH3 or 5-FC1. This mechanism of the resistance of the compound was not completely comprehended. However, several possibilities exist according to the research data. These resemble the upstype 2 mutant that was reported in previous research. The latter isolated the resistance of both the compounds of 5-FC and 5-FU. These indicated the reason for the research. It did not have any impairment in the incorporation of the 4C-labeled cytosine within the trichloroacetic acidinsoluble fraction. The combined deficiencies were emphasized inside the uridine kinase. This was an enzyme that was catalyzing the conversion of the uridine to UMP.
代写论文：化学
The UMPase was accounting for the pattern of the resistance that was viewed in the ups-type 2 mutant. Another explanation was based on the fact that 5-FC was converted into to 5 – fluoro – 2′ – deoxyuridine – 5′ – monophosphate (5F-dUMP) in the 52R. They selectively bind to the thymidylate synthetase (TS) enzyme. This does not incorporate with the RNA within the nucleotide linkage. The 5F-dUMP is a noncompetitive inhibitor that catlysis the methylation process of deoxyuridylic acid to thymidylic acid. This inhibits the DNA synthesis. The 5F-dUMP-enzyme complex known as the trichloroacetic acid is used to stimulate the de novo synthesis of TS. These permit the DNA synthesis to proceed. This occurs at a lower rate. This blocks the actions of the 5-FC. The hypothesis is supported based on the reduced growth rate of the 52R within the presence of the 5-FC. The third explanation is taking into account the drug resistance in the 52R of the 5-FC. From analysis, it is found to be incorporated into the RNA or the 52R compound. These did not cause significant alteration to the cell functions. These situations are probable. 5-FC or 5-FU compound can be defluorinated by 52R and can be accounted for the drug resistance and appearing of the radiolabel in the tricholroacetic acid fraction. These mechanisms are remote and do not have the precedent for the microbiological system. The research indicated the plausible impacts that would arise in the cells based on the interaction between the pathogen and the anti-fungal agent. It is imperative to understand that out of the 6 isolates, 5 isolates had determined in a specific impact. The sixth isolate also explained about the impacts of these relationships. In reality, the structural formation cannot be determined. A broad perspective can be developed based on the research.
